WebThermo Scientific Bacteriophage T7 RNA polymerase is a DNA-dependent RNA polymerase with strict specificity for its respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on either single … WebSP6 or T7 RNA polymerase promoter. This DNA produces a transcript approximately 1,800 bases long. Since luciferase must be full-length to show activity, transcription and translation of the Control DNA followed by a luciferase assay is a convenient means to verify that full-length transcripts have been generated.!
Promoter specificity determinants of T7 RNA polymerase PNAS
Web9 rows · T7 and SP6 RNA polymerases are DNA dependent RNA polymerases that … WebA simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure is described. This in vitro transcription system is based on the unusually specific RNA synthesis b michael fabricant bald
T7 RNA polymerase - Wikipedia
T7 polymerase is extremely promoter-specific and transcribes only DNA downstream of a T7 promoter. The T7 polymerase also requires a double stranded DNA template and Mg ion as cofactor for the synthesis of RNA. It has a very low error rate. T7 polymerase has a molecular weight of 99 kDa. Promoter … See more T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5'→ 3' direction. See more T7 polymerase has been crystallised in several forms and the structures placed in the PDB. These explain how T7 polymerase binds to DNA and … See more In biotechnology applications, T7 RNA polymerase is commonly used to transcribe DNA that has been cloned into vectors that have two (different) phage promoters (e.g., T7 and T3, or T7 and SP6) in opposite orientation. RNA can be selectively … See more • Martin CT, Esposito EA, Theis K, Gong P (2005). "Structure and function in promoter escape by T7 RNA polymerase". Progress in Nucleic Acid Research and Molecular Biology. … See more T7 polymerase is a representative member of the single-subunit DNA-dependent RNAP (ssRNAP) family. Other members include phage T3 and SP6 RNA polymerases, the mitochondrial RNA polymerase (POLRMT), and the chloroplastic … See more • T7 expression system See more • T7 RNA Polymerase Enzymology • OpenWetWare See more WebT7 RNA polymerase transcription initiation site 1 multiple cloning region 10–128 SP6 RNA polymerase promoter (–17 to +3) 139–158 SP6 RNA polymerase transcription initiation site 141 pUC/M13 Reverse Sequencing Primer binding site 176–197 lacZ start codon 180 lac operator 200–216 β-lactamase coding region 1337–2197 phage f1 region 2380–2835 WebBacteriophage T3 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T3 phage promoters. The 99 KD enzyme catalyzes in vitro RNA synthesis from a cloned DNA sequence under the T3 promoters. RNA produced using the T3 RNA Polymerase is suitable for many applications in research and biotechnology. Product … how to change data pivot table