Phospho-yap ser127 antibody

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August undefined, 2024

WebJan 17, 2024 · Phospho (Ser127) View Product On Supplier's Website Request a Quote from Cell Signaling Technology Supplier provided information Immunogen Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP protein. Validations Biological Strategies … WebImmunoprecipitates from HEK293T cell lysates with indicated antibody were blotted with anti-YAP or anti-NLK antibody. EMBO reports Vol 18 No1 2024 ª 2016 The Authors ... Flag-NLK-WT was transfected and phospho-peaks of YAP at Ser127 when Flag-NLK-KM was transfected (Fig EV2). This finding prompted us to explore whether or not NLK affects …

Phospho-YAP (Ser127) Antibody Cell Signaling Technology

WebPhospho (Ser127) View Product On Supplier's Website Request a Quote from Cell Signaling Technology Supplier provided information Immunogen Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP. WebBuy rabbit polyclonal antibody to YAP1 (A94632). Applications: WB, IHC and more. Reactivity: Human, Mouse and more. ️ Low Prices ️ 100% Guarantee slow cooker wedding soup recipe

Osmotic stress‐induced phosphorylation by NLK at Ser128 activates YAP …

WebAntibody Company Cat. No Clonal Dilution IF WB IHC IP pan 14-3-3 Santa cruz sc-1657 monoclonal (clone H-8) 1:2000 Beta-Tubulin Sigma T7816 monoclonal (clone SAP.4G5) ... phospho-YAP (ser127) cell signalling #4911 phospho-YAP (Y357) abcam ab62751 Phospho-Src Family Kinases (Y416) #2101 S100A4 (FSP1) ab27957 Smooth muscle actin … WebProduct Name: Phospho-YAP (Ser127) (D9W2I) Rabbit mAb Product Type: Monoclonal Antibody Species of Origin: Rabbit Lot Number: 6 Concentration: 549 µg/ml Approved Applications: •Immunohistochemistry (Paraffin) •Immunoprecipitation •Western Blotting Approval: Production: Valerie Goss Date: 22-December-2024 WebPhospho-YAP1 (Ser127) Antibody (PA5-117322) in WB Western blot analysis of Phospho-YAP1 (Ser127) in Untreated (–) and treated (+) HeLa whole cell extracts (30 µg). Samples were separated with 10% SDS-PAGE and incubated in Phospho-YAP1 (Ser127) polyclonal antibody ( Product # PA5-117322) using a dilution of 1:5,000. Product Details slow cooker where to buy

Rabbit Anti-Non-phospho YAP (Ser127) Recombinant Antibody …

Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody …

WebPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP. Antibodies are purified by … WebSep 23, 2016 · The protein expression of YAP and p-YAP (Ser127) in Panc-1 exposed to resveratrol and gemcitabine was evaluated by Western blot analysis. As shown in Figure 6B, resveratrol treatment up-regulated the level of p-YAP (Ser127), and the total level of YAP was significantly inhibited by resveratrol. However, gemcitabine had no effect on YAP … slow cooker white bean and chicken chiliWebHere, we describe a novel approach to detect both the relative amount of total YAP (T-YAP assay) and the phosphorylation of the residue S127 of YAP (S127-P-YAP assay) using a HTRF ®-based method. This easy-to-run method can easily be miniaturized and allows for a high-throughput analysis in 96/384-well plate format, requiring less cellular ... soft tubs of maine

"WebThe Phospho-YAP (Ser127) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to YAP1, and Yes1 associated transcriptional regulator. This antibody recognizes Human, Mouse, and Rat antigen. The Phospho-YAP (Ser127) Antibody has been validated for the following applications: Western Blot. " - Phospho-yap ser127 antibody

Phospho-yap ser127 antibody

Phospho-YAP1 (Ser127) Polyclonal Antibody - Thermo …

WebInvitrogen Anti-Phospho-YAP1 (Ser127) Polyclonal, Catalog # PA5-114675. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (PFA fixed) … WebPhospho-YAP (Ser127) Antibody detects endogenous levels of YAP only when phosphorylated at serine 127. Species Reactivity; Human, Mouse, Rat; Product Property. Format; Liquid; Buffer; Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Storage; Shipped at 4°C. Upon delivery aliquot and store at -20°C.

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WebDec 19, 2024 · The level of phosphorylation of the residues serine 127 (S127) of YAP is generally assessed in cellular models, native tissues, and organs, as a marker of YAP activity and location, and is regulated by numerous partners. This phosphorylation event is classically detected using a western blot technical approach. WebPhospho-YAP1 (Ser127) Antibody (PA5-17481) in WB. Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with …

WebA huge increase of YAP1 protein and YAP phosphorylation at Serine 127 (p-YAP Ser127) in HD over LD was detected. We also observed that DUSP10 overexpressing CRC cell lines, HT29-DUSP10 and HCT116-DUSP10, showed higher levels of YAP1 and p-YAP Ser127 than EV cells in LD, that were further augmented by HD (Figure 2d, Figure S3d). WebNov 30, 2024 · YAP1 Antibody (63.7) is an IgG2a κ mouse monoclonal YAP antibody suitable for the detection of YAP of mouse, rat and human origin by WB, IP, IF, IHC (P) and ELISA. YAP1 Antibody (63.7) is available as the non-conjugated anti-YAP antibody form.

WebPhospho-YAP (Ser127) Antibody detects endogenous levels of YAP only when phosphorylated at serine 127. This antibody may cross-react with phospho-TAZ (Ser89). … WebWestern blot analysis of extracts from HeLa cells (lane 1) or YAP knock-out cells (lane 2) using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb #13008 (upper), and GAPDH (D6H11) XP ® Rabbit mAb #5174 (lower). The absence of signal in the YAP knock-out HeLa cells confirms specificity of the antibody for YAP.

WebPhospho-YAP1 (Ser127) Polyclonal Antibody Product Details Size 100 µL Species reactivity Human, Mouse, Non-human primate, Rat Host / Isotype Rabbit / IgG Class Polyclonal Type Antibody Conjugate Unconjugated Immunogen A synthesized peptide derived from human YAP around the phosphorylation site of Ser127. Form Liquid Concentration 1 mg/mL

WebJun 18, 2024 · The fixed cells were stained with an anti-YAP (1:400, sc-271134, Santa Cruz) antibody in 2% BSA for an hour at room temperature and then with Alexa Fluor ® 488-conjugated goat anti-mouse IgG as... soft tubs hot tubs chemicalsWebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) Research with confidence – consistent and … soft tub spa repairWebMonoclonal Antibody for studying YAP (Ser127) phosphate. Cited in 316 publications. Validated for WB, IP, IHC. Available in 2 sizes. Highly specific and rigorously validated in … soft tubs hot tubs partsWebThe Phospho-YAP (Ser127) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to YAP1, and Yes1 associated transcriptional regulator. This antibody … soft tufted ottomanWebImmunofluorescence analysis of Hela cell. 1, YAP (phospho Ser127) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). soft tub victoria bc soft tub spa suppliesWebJan 10, 2024 · The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity … soft tub spa pack