Phis2
Webb1 apr. 2024 · To investigate the binding of TaMYB44-D to starch biosynthesis-related genes, the promoter sequences of 12 selected genes were cloned in front of the reporter gene HIS3 to construct the pHIS2.1-Pro plasmids. The plasmids pHIS2.1-Pro and pGADT7-Sec2-TaMYB44-D were co-transfected into the Y187 yeast strain to perform the Y1H … Webb8 juni 2014 · 本研究根据植物胚柄特异表达基G564启动子关键序列(GAAAAGCGAA)合成次串联重复序列，并与报告质粒pHIS2．1连接构建诱饵载体pHIS2．1．G564．5A；以纯化的拟南芥rabidopsisthaliana)幼嫩种子mRNA模板，合成SMARTTM和CDS1I锚定末端dscDNA：并将dscDNA与线性化融合表达载体pGADT7一Rec2 ...
Phis2
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Webb16 feb. 2024 · The product was cloned into the pHIS2 vector for the bait construction. The coding sequence of PhOBF1 with full length was introduced into the pGADT7-Rec vector harboring the GAL4 activation domain, which was regarded as the pray construct. The recombinant plasmids were co-transformed into Saccharomyces cerevisiae strain Y187. WebbSnapGene Viewer lets you see your data for free with the same rich visualization, annotation, and sharing capabilities as the fully enabled SnapGene software. SnapGene …
Webb29 feb. 2024 · The empty vector pGADT7-Rec2 and the pHIS2-cis/promoter were co-transformed as the control for mating experiments. DNA–protein interactions were … Webb6 feb. 2024 · The chloroplast comprises the outer and inner membranes that are composed of the translocon protein complexes Toc and Tic (translocon at the …
Webb1 dec. 2024 · AD/pATM1-pHIS2 and AD-ATM1/pATM1-pHIS2 were cotransformed into the Y187 strain and cultured on SD/-Trp/-Leu for 2–3 days at 30 °C. Then, healthy and … Webb6 okt. 2024 · Furthermore, the AD-TFs and pHIS2- CmRAP2.3pro were co-expressed in Y187 in the SD/-His/-Leu/-Trp medium at 30°C for 3 days. Yeast cells harbouring AD vector and pHIS2-CmRAP2.3pro were used as the negative control. These yeast cells were incubated in SD/-His-Leu-Trp medium with 80 m m 3-AT at 30°C for 3 days. Luciferase …
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Webb12 apr. 2024 · 新-酵母单杂交筛选与启动子phis2-a互作蛋白结题报告; 配套k122024届高考化学一轮复习 选考 有机化学基础 第1节 有机化合物的组; 职业技术学院智慧校园系统需求规划知识讲解; 幼儿园小班教案《美丽花园》含反思; 档案数字化服务外包规范; 环境保护行政复议 … read my lips challenge statementsWebb1 dec. 2024 · d Growth of yeast cells on SD/-Trp-Leu-His supplemented with 100 mM 3-AT. pHIS2.1-ProGA2ox3 plus pGADT7 and pHIS2.1-ProGA2ox7 plus pGADT7 served as the negative controls. read my lips mechanicsWebbPhis2 1 Bait Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … how to stop sprinkler head from leakingWebbxho1和sac1的酶切位点是什么？ 它识别的特定基因是体内3广a泛存在的siRNA和miRNA前体。Dicer酶的PAZ结构域特异性地识别 0’端有 5个j碱基突出的 dsRNA,之w后由RNase III结构域剪切8生成siRNA或microRNA。 2011-10-27 8:58:59 read my lips full movie watch onlineWebbOur dedication to customer support brings unique and effective solutions to your specific challenges and applications. Partnering with Takara Bio allows you to: Collaborate with … read my lips game zoomWebbpHIS2.1 DNA target TRP1 Kanr HIS3 Figure 2. One-hybrid library construction and screening. Your target bait sequence is cloned upstream of the His3 reporter in pHIS2.1. … how to stop sql injection attacksWebb19 sep. 2024 · These sequences were cloned into pHIS2 (Clontech, Palo Alto, CA, USA) upstream of the reporter gene HIS3. Co-transformation of pHIS2 with or without the TCP cis-element sequences and GbTCP4-pGADT7 into Y187 cells was performed as described by Yu et al. (2024) . The pGADT7 vector carries the LEU2 nutritional marker for selection … read my lips no new taxes quote