Webbvector pHis1525 19. FliCD was expressed in B. megaterium and puried as described previously 20. Preparation of C. difficile spores Sporulation of the C. difficile R20291 strains were induced in Clospore medium as described previously 21. Briefl y, an overnight 20 ml of C. difficile cultured in Columbia Broth was inoculated into 500 ml of WebbpHIS1525 (Mobitec GmbH, Göttingen, Germany; Table 2). The RBS of P xylA as optimized by Malten et al. (2005)was introduced by PCR amplification from pHIS1525 by miss-matching positions in the reverse primer Pxyl_RBS+ _Gibson_rv, thereby generating P xylA+. The oligonucleotide primers used in this study are listed in Supplementary

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WebbExpression of keratinase gene in Bacillus megaterium using an expression vector of pHIS1525.SP lipA and utilization of the resulting recombinant strain for chicken feather … WebbIn order to develop a stable strain of Bacillus megaterium for Keratinase production, the Keratinase gene (KerA) of Bacillus lichiniformis ATCC 53757 and SPlipA gene from plasmid pHIS1525.SPlipA (B ... porch swing hardware home depot

Holdings: A pathogenic PSEN2 p.His169Asn mutation associated …

WebbThis research showed that the b-xylosidase gene was succesfully sub-cloned in pHIS1525 system and expressed by the recombinant B. megaterium MS941. Theaddition of 0.5% … WebbSee also. Characterisation of a recombinant β-xylosidase (xylA) from Aspergillus oryzae... Characterisation of a recombinant β-xylosidase (xylA) from Aspergillus oryzae expressed in Pichia pastoris WebbDownload dengan 2 jenis enzim restriksi SacI dan SphI, (2) rekombinasi. (ligasi) amplikon dan plasmid pHIS1525 menggunakan enzim ligase (3) transformasi plasmid ... porch swing hanging rope