WebOct 31, 2016 · For immunocytochemistry of BMDM, coverslips with adherent macrophages were removed from the plate well. Cells were fixed with Cytofixx cell spray (CellPath) for 30 min at room temperature and with 4% PFA for another 30 min. To block unspecific antibody binding, cells were incubated with 5% FCS in PBS for 1 h at room temperature. WebCytoFixx-II are ready to use solutions containing alcohol and carbowax, offering consistent fixation results and subsequent staining. Pump Spray in 50ml (enough to fix approximately 70 slides) and a more economical 150ml (enough to fix approx 205 slides). Fixative is dispensed in a fine controlled spray with a simple pump action.
Cytological Fixative Cytofix Aerosol 150ml - approx 300 doses
CytoFixx is a CFC free, ready-to-use solution containing alcohol and carbowax, offering consistent fixation results and subsequent staining. The pump spray enables the fixative to be directed onto the microscope slide in a more controlled manner.Key Benef WebCytofixx Pump Spray 50ml [Pack of 10] Availability: Out of stock For further enquiries please contact [email protected] GPS Price £31.00 £37.20 Notify me when the … ct winnipeg
A vaccine targeting mutant IDH1 induces antitumour immunity
WebSpray CYTOFIX® on the slide with a smear for a short time (ca 0.5 s) from the distance of approximately 30 cm. Put the slide away on the level surface until it dries. After a few minutes, the preparation is properly fixed and protected - can be coloured, diagnosed or prepared for transport. Tags: Standard equipment , Cytology WebFixation was done using 30 min Cytofixx Pump Spray cell path and 30 min 4% (vol/vol) para- formaldehyde. For detection the Red Duolink In Situ Proximity Ligation Assay (PLA) Kit was performed according to manufacturer´s instructions with anti-NDRG1 polyclonal rabbit (Sigma-Aldrich) and mouse anti-MGMT (Life Technologies) applied for 12 h. WebCytology Fixative combines a convenient fine-mist spray dispenser with a polyethylene glycol formulation to enable the rapid, high quality fixation of cytology cell spreads. … ctwinnipeg.com